UC San Diego Biologists Discover
|Regrowing axons 12 hours (left) and 24 hours (right) after injury. Credit: Lizhen Chen, UC San Diego|
“There are many processes not involved in early development that are involved in switching the neurons to this re-growth mode,” said Chisholm. “In essence what we found are genes that people had not suspected previously to be part of this process.”
Of particular interest to the UC San Diego biologists are the six genes that appear to repress the growth of axons.
“The discovery of these inhibitors is probably the most exciting finding,” said Chisholm, because identifying and eliminating the inhibiting factors to the re-growth of axons could be just as essential as the biochemical pathways that promote axon re-growth in repairing spinal cord injuries and other kinds of nerve damage.
The scientists were also surprised to learn that some of the genes they found to be involved in the re-growth of axons were known to have other functions, such as regulating the release of neurotransmitters.
“This was in large part unexpected,” said Chisholm. “These genes had not been implicated in the re-growth of axons before.”
To find the 76 genes, the researchers conducted painstaking experiments on more than 10,000 tiny laboratory roundworms known as C. elegans. The first step involved developing genetic mutants of these transparent roundworms for each one of 654 genes that were suspected to play a role in the regulation of axon regrowth in worms, fruit flies and mice. They then labeled the roundworm neurons with green fluorescent protein and, with a precise surgical laser, damaged a specific axon.
“The goal was to study this process in its simplest form,” said Chisholm. “Because the animals are essentially transparent, we can see the axons expressing this green fluorescent protein.”
By examining the re-growth, or lack of growth, of the damaged axon 24 hours later, the scientists were then able to determine which of these 654 genes were actually important to axon re-growth.
Chisholm said that while the 76 genes identified are believed to have similar roles in mammals as well as roundworms, because their functions were “conserved” by the organisms through evolution, he and his research team are now collaborating with other investigators to conduct experiments on mice to verify this connection and determine which of these genes are the most critically important.
“Worms are clearly different from mammals,” he added. “But there will be a core of conserved molecules doing the same job.”
In addition to Chisholm and Jin, the UC San Diego biologists involved in the study were Lizhen Chen, Zhiping Wang, Anindya Ghosh-Roy, Thomas Hubert, Dong Yan, and Zilu Wu. Sean O’Rourke and Bruce Bowerman from the University of Oregon were also part of the team.
The research project was supported by grants from the National Institutes of Health and the Howard Hughes Medical Institute.
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Comment: Andrew Chisholm (858) 534-7783, email@example.com